Probe 96 Experimental Conditions in a Single Run
Each channel of the CFM can be a different solution, enabling up to 96 different experimental conditions to be probed in a single experiment.
Because the ligand deposition process is done using the CFM’s enclosed microchannels, each ligand spot can have a different concentration, buffer composition, pH, detergent mix, and so forth. Thus, 96 different conditions can be assayed in a single step, while the protein of interest is still active.
For biosensor work this could include the optimization of:
- Buffer composition
- Coupling conditions
- Capture conditions
- Solubilization detergents
- Regeneration conditions
For example, in prior work studying GPCR solubilization conditions, we optimized the solubilization of CXCR5 in 2 hours by evaluating 96 different solubilization cocktails, while the same process would have up to a week on a standard SPR biosensor and 96 changes of the system running buffer.
